Quantitative airborne spore traps' analysis of the spatial distribution of the hocks

The spatiotemporal dynamics of Sporozoa sporangia proliferation in grapevines in China is rarely reported. For this purpose, the temporal and spatial dynamics of sporangia dissemination of Pseudoperonospora cubensis were observed by a quantitative airborne spore trapping method, combined with a correlation analysis of field disease system investigations and the amount of spores detected, to understand the sporangia diffusion dynamics and field conditions. The relevance of the disease condition, which in turn provides the basis for the occurrence of the disease and its forecast and forecast. The quantitative airflow spore traps are suspended at 1.5m and 2.5m respectively. The slide coated with a thin layer of Vaseline is placed on the spear catcher. On the slide holder, open the spore catcher to collect the spores. The inspection method is the same as in 1.3.1. The number of sporocysts captured at 1.5m and 2.5m was added, and the average number was calculated as the number of sporangia captured during 15 min of this period. Then, the number of sporangia captured at different time points was plotted on the vertical axis, and the time was taken as the The horizontal axis shows the curve of the amount of spores at different times of the day.

The spore sac was captured at different heights in the orchard from 0 to 6 m by quantitative air spore traps. It was found that the number of sporangia captured near the ground was generally the highest in the absence of rain, and the number of sporangia was regular with increasing height. Spontaneously reduced, the sporangium of Peronosporium can still be captured at 6m, but the number has decreased significantly. At the 2.5 m canopy, the number of sporangia did not decrease at 2 m, but increased. This may be consistent with the height of the sporangia and the height of the scaffold. It is the leaf layer that is relatively concentrated, especially the young leaves with strong susceptibility. More focused on.

During sporulation, slides coated with a thin layer of Vaseline were placed on a slide holder of a quantitative airflow spore catcher. The slide holders were then pushed forward, and the power was switched on for capture, each time for 10 min. After the slide was returned to the laboratory, the sporangia were examined and counted. The average amount of spores at each height was plotted on the vertical axis, and the height of the capture was plotted on the horizontal axis. The captured amount of spores at different heights was compared. .

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